As long as 1/5, 1/10 or even 1/20 of the samples and reagents used in traditional library construction, DBS-LibPrep can automatically complete fragmentation, end repair and A-tailing, adapter ligation, library amplification (PCR), and bead cleanup. Users only need to add samples and reagents, and other steps are automatically completed on the chip. The KAPA library preparation can be completed in 1.5 hours. DBS-LibPrep is flexible and suitable for different library preparation methods.

The following is the flowchart of running KAPA HyperPlus Library Prep Kit on DBS-LibPrep. Currently, the reaction volumes have been reduced to 5%, but the reaction steps and times are kept the same as the original protocol. Optimization will be done next to reduce the overall library prep time.

Instrument Operations:

A touch screen is provided to make the instrument easy to use. Small volumes of samples and reagents are loaded to a DMF chip. All library preparation steps are performed on the DMF chip in a DBS-LibPrep instrument. Only 5 minutes of hands-on time is needed to set up an experiment.

DBS-LibPrep for Library Preparation On-chip and Off-chip Performance Comparison

Reagent Kit：Kapa HyperPlus Library Preparation Kit

Reaction volume: 1/20 of the recommended volume in the Kit

Reaction time：Same as the recommended in the Kit

Experiments are carried out to verify the performance of using a digital microfluidic chip for library preparation, in which the reaction volume is reduced to 1/20 of the conventional volume. Reaction time is kept the same for easier comparison purposes. The input DNA (12.5ng) is provided by a collaborator. On-chip and off-chip performance results are compared for all the individual library preparation steps. The Agilent 2100 measurements show good consistency between on-chip and off-chip library preparation.

1. ERAT+Ligation – On-chip and off-chip performance comparison

2. After Ligation Bead Cleanup – On-chip and off-chip performance comparison

3. PCR – On-chip andoff-chip performance comparison

Next generation sequencing (NGS) has become a key technology in many areas of biology and other sciences such as medicine and forensics, and high-quality library preparation is a key to a successful sequencing.

Presented here is an automated library prep system, including instrument and device, based on droplet microfluidics technology. The desktop instrument has included functions such as temperature control, magnetic beads cleaning, and amplification, etc., making automated library prep possible without the need for expensive liquid handlers.

This DBS-LibPrep system has been validated using KAPA HyperPlus Library Prep Kit and a few other kits. DBS provides customized devices for your special library prep needs.

DBS-LibPrep Characteristics

System in Sum

Integrated – All library prep steps are integrated and automated

Easy to Use – System has been calibrated at factory. Easy installation and operation

Modern Design – Glass window on the top and 10-inch touch screen

Reduced Hands-on Time – 80% less than manual library prep

Reduced Reagent Usage – 5% - 10% of the standard protocol volume

Repeatability – High quality and repeatable libraries

Customization – System can be customized to suit specific library prep needs

Open the instrument lid, insert the device, load samples and reagents, close the lid, and press the start button on the touch screen, the instrument will finish the library prep without further user interaction. Currently, up to 4 samples can be run simultaneously.

The following is the flowchart of running KAPA HyperPlus Library Prep Kit on DBS-LibPrep. Currently, the reaction volumes have been reduced to 5%, but the reaction steps and times are kept the same as the original protocol. Optimization will be done next to reduce the overall library prep time.